bs-70280R

DATASHEET

Host: Rabbit

Target Protein: Tau (Ser416)

Specificity: Specific for ~59, 65, 68 kDa tau protein phosphorylated at Ser416. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser416

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 69329

Swiss Prot: P19332

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of rat tau, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Ser-416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 59/65/68

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.