bs-70276R

DATASHEET

Host: Rabbit

Target Protein: Synaptotagmin (Ser309)

Specificity: Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser309

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25716

Swiss Prot: P21707

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 60/62

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Bovine
Chicken
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.