bs-70261R

DATASHEET

Host: Rabbit

Target Protein: SNAP25 (Ser187)

Specificity: Specific for endogenous levels of the ~25 kDa SNAP25 protein phosphorylated at Ser187. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser187

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25012

Swiss Prot: P60881

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 of rat SNAP25, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on Ser-187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since Ser-187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 25

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Guinea Pig
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.