bs-70258R

DATASHEET

Host: Rabbit

Target Protein: Serotonin Transporter (Thr276)

Specificity: Specific for endogenous levels of the ~76 kDa SERT protein phosphorylated at Thr276. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Thr276

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25553

Swiss Prot: P31652

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 of rat SERT, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at Thr-276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at Thr-276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the Thr-276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 76

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Bovine
Sheep
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.