bs-70243R

DATASHEET

Host: Rabbit

Target Protein: REDD1 (Thr23/25)

Specificity: Specific for endogenous levels of the ~34 kDa REDD1 phosphorylated at Thr23/25. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Thr23,25

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54541

Swiss Prot: Q9NX09

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 of human REDD1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr-25, Thr-23 and Ser-19 through the activity of GSK3b (Katiyar et al, 2009).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 34

Cross Reactive Species: Human

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.