bs-70241R

DATASHEET

Host: Rabbit

Target Protein: Raf-1 (Ser642)

Specificity: Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser642. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser642

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24703

Swiss Prot: P11365

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser-301 and Ser-642.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 74

Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Bovine
Chicken
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.