bs-70239R

DATASHEET

Host: Rabbit

Target Protein: Rabphilin 3A (Ser234)

Specificity: Specific for endogenous levels of the ~82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser234

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 171039

Swiss Prot: P47709

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat rabphilin 3A, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 82

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.