bs-70236R

DATASHEET

Host: Rabbit

Target Protein: PTPH1 (Ser459)

Specificity: Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser459

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5774

Swiss Prot: P26045

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 104

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Dog
Bovine
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.