bs-70229R

DATASHEET

Host: Rabbit

Target Protein: Potassium Chloride Cotransporter (KCC2) (Thr1007)

Specificity: Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Thr1007. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr1007

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 171373

Swiss Prot: Q63633

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1007 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser-940 residue, while decreasing phosphorylation of Thr-1007 residue. Dephosphorylation of residues Thr-906 and Thr-1007 correlates with increased KCC2 activity (Moss et al., 2017).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 135

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Bovine
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.