bs-70217R

DATASHEET

Host: Rabbit

Target Protein: Parkin (Ser378)

Specificity: Specific for the ~52 kDa parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.

Modification Site: Ser378

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5071

Swiss Prot: O60260

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 of human Parkin, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substantia nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser-378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 52

Cross Reactive Species: Human

Predicted Cross Reactive Species: Bovine
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.