bs-70214R

DATASHEET

Host: Rabbit

Target Protein: PanK4 (Thr63)

Specificity: Specific for the ~86 kDa PanK4 phosphorylated at Ser63. Immunolabeling of PanK4 is nearly eliminated with λ-phosphatase treatment.

Modification Site: Thr63

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 269614

Swiss Prot: Q80YV4

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr63 of mouse PanK4, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967). There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001). PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia. PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001). Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 86

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Rat
Hamster

For research use only. Not intended for diagnostic or therapeutic use.