bs-70212R

DATASHEET

Host: Rabbit

Target Protein: PAK-1 (Thr84)

Specificity: Specific for endogenous levels of the ~68 kDa PAK1 phosphorylated at Thr84. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr84

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5058

Swiss Prot: Q13153

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 of human PAK1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at Thr-84 and bind to gelsolin (Chen et al, 2004).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 68

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Sheep
Chicken
Guinea Pig
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.