bs-70211R

DATASHEET

Host: Rabbit

Target Protein: p62 (Thr269, Ser272)

Specificity: Specific for endogenous levels of the ~62 kDa p62 protein phosphorylated at Thr269 and Ser272. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Thr269, Ser272

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8878

Swiss Prot: Q13501

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr269/Ser272 of human p62, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The protein scaffold and signaling regulator p62 (also known as sequestosome1 (SQSTM1)) is important in critical cellular functions, including bone homeostasis, obesity, and cancer, because of its interactions with various signaling intermediaries. p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at Thr-269 and Ser-272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Moscat et al., 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation (Moscat et al., 2011).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 62

Cross Reactive Species: Human

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.