bs-70208R

DATASHEET

Host: Rabbit

Target Protein: p38 MAPK (Thr180/Tyr182)

Specificity: Specific for endogenous levels of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Thr180, Tyr182

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 70496

Swiss Prot: P70618

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 of rat p38/MAPK, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002; Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr-180 and Tyr-182 (Lin et al., 1995).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 39

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Bovine
Chicken
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.