bs-70207R

DATASHEET

Host: Rabbit

Target Protein: Olig2 (Ser10/13/14)

Specificity: Specific for endogenous levels of the ~32 kDa Olig2 phosphorylated at Ser10, Ser 13, and Ser14. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser10,13,14

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10215

Swiss Prot: Q13516

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 of human Olig2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 32

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Guinea Pig
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.