bs-70199R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR2C Subunit (Ser1096)

Specificity: Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser1096. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser1096

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24411

Swiss Prot: Q00961

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1096 of the NR2C subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser-1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009)

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 140

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.