bs-70196R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR2B Subunit (Tyr1472)

Specificity: Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Tyr1472

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24410

Swiss Prot: Q00960

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr-1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 180

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.