bs-70195R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR2B Subunit (Tyr1336)

Specificity: Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1336 . Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Tyr1336

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24410

Swiss Prot: Q00960

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr-1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 180

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.