bs-70194R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR2B Subunit (Tyr1252)

Specificity: Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with λ-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.

Modification Site: Tyr1252

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24410

Swiss Prot: Q00960

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr-1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 180

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.