bs-70186R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR2A Subunit

Specificity: Specific for endogenous levels of the ~180 kDa NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24409

Swiss Prot: Q00959

Source: Fusion protein from the C-terminal region of the NR2A subunit of the rat NMDA receptor.

Purification: Neat Pooled whole antiserum

Storage Buffer: Neat whole antiserum

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.