bs-70167R [Primary Antibody]
NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: NCC (Thiazide sensitive NaCl cotransporter)

Specificity: Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.

Modification Site: Thr53

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20497

Swiss Prot: P59158

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 160


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Guinea Pig
Hamster

For research use only. Not intended for diagnostic or therapeutic use.