bs-70166CH

DATASHEET

Host: Chicken

Target Protein: Myelin Basic Protein (MBP)

Specificity: Specific for endogenous levels of the ~18 kDa & ~22 kDa myelin basic protein.

Clonality: Polyclonal

Isotype: IgY

Entrez Gene: 24547

Swiss Prot: P02688

Source: Three peptide sequences conserved in higher vertebrate MBP proteins

Purification: Total IgY fraction

Storage Buffer: PBS + 10 mM NaN3.

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 18/22

Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.