bs-70165R

DATASHEET

Host: Rabbit

Target Protein: Munc-18 (Ser 513)

Specificity: Specific for endogenous levels of the ~65 kDa Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser513

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 81804

Swiss Prot: Q62753

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996). Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 65

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Bovine
Chicken
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.