bs-70162R

DATASHEET

Host: Rabbit

Target Protein: Mnk2a

Specificity: Specific for endogenous levels of the ~52 kDa Mnk2a protein. Immunolabeling is blocked by preadsorption with the Mnk2a peptide used as antigen, but not by Mnk2b peptide.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2872

Swiss Prot: Q9HBH9

Source: Synthetic peptide from the C-terminal region, specific to the human Mnk2a isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2b.

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

MAP kinase-interacting kinases Mnk1 and Mnk2 are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997). Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997). Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005). Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 47

Cross Reactive Species: Human

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.