bs-70158R

DATASHEET

Host: Rabbit

Target Protein: Metabotropic Glutamate Receptor 5/1a

Specificity: Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa dimers of mGluR5 and mGluR1. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24418

Swiss Prot: P31424

Source: Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat mGluR5 and rat mGluR1a, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 125/250

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.