bs-70155R

DATASHEET

Host: Rabbit

Target Protein: MEK5 (Ser311, Thr315)

Specificity: Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser311, Thr315

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 31068

Swiss Prot: Q62862

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 49

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.