bs-70153R

DATASHEET

Host: Rabbit

Target Protein: MEK1 (Thr292)

Specificity: Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr292.

Modification Site: Thr292

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5604

Swiss Prot: Q02750

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr-292 and Thr-386 (Mansour et al., 1994).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 45

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Bovine
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.