bs-70149R

DATASHEET

Host: Rabbit

Target Protein: MeCP2 (Ser80)

Specificity: Specific for endogenous levels of the ~75 kDa MeCP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.

Modification Site: Ser80

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4204

Swiss Prot: P51608

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 of human MeCP2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: whole antiserum

Storage Buffer: whole antiserum

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)