bs-70145R

DATASHEET

Host: Rabbit

Target Protein: MARCKS (Ser152,156)

Specificity: Specific for endogenous levels of the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser152,156

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3028

Swiss Prot: P30009

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser-152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994)

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 87

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Bovine
Chicken
Zebrafish
Xenopus

For research use only. Not intended for diagnostic or therapeutic use.