bs-70134R

DATASHEET

Host: Rabbit

Target Protein: Interferon-α Receptor, Type I, Subunit I (Ser535,539)

Specificity: Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 kDa - 130 kDa.

Modification Site: Ser535,539

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3654

Swiss Prot: P17181

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine-535 and Ser-539 in the IFNAR1 (Kumar et al., 2003).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 110-130

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Bovine
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.