bs-70130R

DATASHEET

Host: Rabbit

Target Protein: HDAC2 (Ser394)

Specificity: Specific for endogenous levels of the ~55 kDa HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser394

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3066

Swiss Prot: Q92769

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of Ser-394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser-422 and Ser-424 (EOM et al, 2011).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 55

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Bovine
Sheep
Chicken
Guinea Pig
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.