bs-70128R

DATASHEET

Host: Rabbit

Target Protein: Glycogen synthase kinase 3 B (GSK3B) (Ser9)

Specificity: Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Modification Site: Ser9

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84027

Swiss Prot: P18266

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 46

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.