bs-70121R

DATASHEET

Host: Rabbit

Target Protein: GluR1-Subunit (Ser831)

Specificity: Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser831. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser831

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 50592

Swiss Prot: P19490

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 of rat GluR1 conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser-831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Soderling and Derkach, 2000).

Size: 150ul

Concentration: Lot Dependent

Predicted Molecular Weight: 100

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Chicken

For research use only. Not intended for diagnostic or therapeutic use.