bs-70116R

DATASHEET

Host: Rabbit

Target Protein: Gap-43 (Ser41)

Specificity: Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser41

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29423

Swiss Prot: P07936

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser-41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 50

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.