bs-70108R

DATASHEET

Host: Rabbit

Target Protein: GABAA Receptor γ2

Specificity: Specific for endogenous levels of the ~46 kDa γ2-subunit of the GABAA receptor.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29709

Swiss Prot: P18508

Source: Synthetic peptide corresponding to amino acid residues specific to the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Neat Pooled whole antiserum

Storage Buffer: Neat whole antiserum

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABA-A-R function (Brandon et al., 2003).

Size: 50ul

Concentration: Lot Dependent

Predicted Molecular Weight: 46

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Chicken
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.