bs-70106R

DATASHEET

Host: Rabbit

Target Protein: GABAA Receptor, ß3 (Ser408/409)

Specificity: Specific for endogenous levels of the ~53 kDa GABAA receptor 3 subunit phosphorylated at Ser408/409. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser408,409

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24922

Swiss Prot: P63079

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 of the ß3 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the β3 subunit have been shown to be critical for the functional modulation of β3 containing recombinant receptors (Brandon et al., 2000).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 53

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.