bs-70097R

DATASHEET

Host: Rabbit

Target Protein: GABAA Receptor α2

Specificity: Specific for endogenous levels of the ~51 kDa α2-subunit of the GABAA receptor in Western blots. Labeling is absent in α2-subunit knockout animals.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 61856

Swiss Prot: P23576

Source: Fusion protein from the cytoplasmic loop of the α2 subunit of rat GABAA receptor.

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channelassociated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABA_A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 51

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Bovine
Zebrafish
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.