bs-70090R

DATASHEET

Host: Rabbit

Target Protein: FMRP (Ser499)

Specificity: Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser499

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24948

Swiss Prot: Q80WE1

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser-499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 71

Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Dog
Bovine
Sheep
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.