bs-70086R

DATASHEET

Host: Rabbit

Target Protein: FAM129B (Ser679, 683)

Specificity: Specific for endogenous levels of the ~83 kDa FAM129B phosphorylated at Ser679/683. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser679, Ser683

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 64855

Swiss Prot: Q96TA1

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser679/683 of human FAM129B, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas (Adachi et al., 2004; Sun et al., 2007). Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response (Sun et al., 2007). FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto’s thyroiditis (Matsumoto et al., 2006). It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion (Old et al., 2009). It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion (Old et al., 2009).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 83

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.