bs-70084R

DATASHEET

Host: Rabbit

Target Protein: ERK/MAPK (Thr202/Tyr204)

Specificity: Specific for endogenous levels of the ~42 - 44 kDa ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr202, Tyr204

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 116590

Swiss Prot: P63086

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 42/44

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.