bs-70082R

DATASHEET

Host: Rabbit

Target Protein: EphrinB (Tyr331)

Specificity: Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr331. Immunolabeling is completely eliminated by treatment with λ-phosphatase. Note: Chicken Tyr331 is the homolog of mouse and rat Tyr343, human Tyr344 and also Xenopus Tyr324.

Modification Site: Tyr331

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 396513

Swiss Prot: P28693

Source: Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 46

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Bovine
Chicken
Zebrafish
Xenopus

For research use only. Not intended for diagnostic or therapeutic use.