bs-70080R

DATASHEET

Host: Rabbit

Target Protein: EphrinB (Tyr298)

Specificity: Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr298. Immunolabeling is completely eliminated by treatment with λ-phosphatase. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.

Modification Site: Tyr298

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 396513

Swiss Prot: P28693

Source: Synthetic phosphopeptide corresponding to amino acid residues surrounding the Tyr298 of Xenopus EphrinB, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 46

Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.