bs-70064R

DATASHEET

Host: Rabbit

Target Protein: DGCR8 (Ser377)

Specificity: Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser377

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1644

Swiss Prot: Q8WYQ5

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010).