bs-70063R

DATASHEET

Host: Rabbit

Target Protein: DENND3 (Ser554)

Specificity: Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser554

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 315055

Swiss Prot: A2RUS2

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 142

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.