bs-70062R

DATASHEET

Host: Rabbit

Target Protein: DENND3 (Thr450)

Specificity: Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Thr450. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr450

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 315055

Swiss Prot: A2RUS2

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 142

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.