bs-70060R

DATASHEET

Host: Rabbit

Target Protein: DARPP-32 (Thr75)

Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr75

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 360616

Swiss Prot: Q6J4I0

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat DARPP-32 conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 32

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.