bs-70057R

DATASHEET

Host: Rabbit

Target Protein: CtIP (Thr847)

Specificity: Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Thr847. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr847

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5932

Swiss Prot: Q99708

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr847 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 100

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Dog
Bovine
Sheep
Chicken
Guinea Pig
Goat
Non-Human Primate
Finch

For research use only. Not intended for diagnostic or therapeutic use.