bs-70051R

DATASHEET

Host: Rabbit

Target Protein: Connexin 43 (Ser368)

Specificity: Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser368

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24392

Swiss Prot: P08050

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 43

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Bovine
Chicken
Guinea Pig
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.