DATASHEET
Host:
Rabbit
Target Protein:
beta Catenin
Specificity:
Specific for endogenous levels of the ~ 92 kDa beta Catenin protein. Immunolabeling of the beta Catenin band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
84353
Swiss Prot:
Q9WU82
Source:
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat beta Catenin, conjugated to keyhole limpet hemocyanin (KLH).
Purification:
Antigen Affinity purification from Pooled whole antiserum
Storage Buffer:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage:
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
beta Catenin, considered a moonlighting protein, is a component of mammalian cell adhesion complex in the cytoplasm along with playing a key downstream component of the canonical Wingless Int-1 (Wnt) signaling pathway in the nucleus (McTrea P.D et al, 1991 and Kemler R. et al, 1993). As part of a protein complex that forms adheren junctions, beta Catenin is necessary for the creation and maintenance of epithelial cell layers and barriers (Brembeck F.H. et al, 2006). beta Catenin is responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete (Brembeck F.H. et al, 2006). The Wnt/beta Catenin signaling pathway plays important roles in mammalian development (Logan C.Y. et al, 2004 ) and irregular regulation of this signaling pathway is associated with human diseases and cancers (Moon R.T., 2004). HIPK2 is a negative regulator of the Wnt signaling via the direct phosphorylation of beta Catenin at ser33/37 causing degradation (Kim E.A. et al, 2010). Additionally, stabilization of beta Catenin has been shown when AMPK phosphorylates at ser552 regulating energy metabolism to cell differentiation and development by enhancing the beta Catenin/TCF mediated transcription within the Wnt/betaCatenin signaling pathway (Zhao J. et al, 2010).