bs-70025R

DATASHEET

Host: Rabbit

Target Protein: Beclin1 (Ser234)

Specificity: Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser234. Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant.

Modification Site: Ser234

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8678

Swiss Prot: Q14457

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser-234 (Wang et al., 2012).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 60

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.